Alice is a zookeeper and the secondary antagonist of The Penguins of Madagascar series. She seems to dislike aspects of her job, and is happy to have a voice-automated robot guide give directions so she can avoid "annoying tour group questions". She also doesn't understand what people enjoy watching animals on their computer, saying that they don't do anything interesting.
Alice is involved in several aspects of the animals' well-being. It's hinted that deep down Alice cares for the Zoo Animals and only wants what's best for them as she hates it when people feed the animals popcorn, and posts several signs throughout the zoo after catching a child feeding Marlene a kernel (though this may be more in fear of losing her job). Alice is also in charge of gathering the penguins for visits to the veterinarian and later remarks that they have a dentist appointment (Private then confusedly states that they don't have teeth).
Alice and the penguins have a mutual dislike for each other, and she believes that they are scheming (though she is actually correct in this assumption).
Though another zookeeper can often be heard over her walkie-talkie, Alice is the only one usually seen on-screen. It is implied that this might be zookeeper Maurice.
Unintentionally, Alice won the zoo's internet popularity contest when a camera documenting the animals was accidentally pushed toward her dancing and slapping her butt in the kitchen.
In Miss Understanding, she remarked to a know-it-all kid that the zoo had three male penguins and one female, which turned out to be false (not that she knew or cared).
She had a best friend, Bella Bon Bueno. (Confirmation needed!)